Anti-IL10 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A00021-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of IL10 using anti-IL10 antibody (A00021-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: rat testis tissue lysates,
Lane 3: mouse testis tissue lysates,
Lane 4: mouse SP20 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (A00021-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL10 at approximately 21 kDa. The expected band size for IL10 is at 21 kDa.
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产品名称

Anti-IL10 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ELISA

基因名称

Il10

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human IL10 recombinant protein (Position: S19-N178).

计算分子量

21 kDa

实际分子量

21 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse Il 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Uniprot ID

P22301

RRID

AB_3080885

文献引用格式

IL10 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00021-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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