Western blot analysis of CD56/NCAM1 using anti-CD56/NCAM1 antibody (A00184-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: Mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD56/NCAM1 antigen affinity purified polyclonal antibody (A00184-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD56/NCAM1 at approximately 120-150 kDa. The expected band size for CD56/NCAM1 is at 95 kDa.
IF analysis of CD56/NCAM1 using anti-CD56/NCAM1 antibody (A00184-4).
CD56/NCAM1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-CD56/NCAM1 Antibody (A00184-4) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of 293T cells using anti-CD56/NCAM1 antibody (A00184-4).
Overlay histogram showing 293T cells stained with A00184-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD56/NCAM1 Antibody (A00184-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Flow Cytometry analysis of U2OS cells using anti-CD56/NCAM1 antibody (A00184-4).
Overlay histogram showing U2OS cells stained with A00184-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD56/NCAM1 Antibody (A00184-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Western blot analysis of CD56/NCAM1 using anti-CD56/NCAM1 antibody (A00184-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: Mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD56/NCAM1 antigen affinity purified polyclonal antibody (A00184-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD56/NCAM1 at approximately 120-150 kDa. The expected band size for CD56/NCAM1 is at 95 kDa.
IF analysis of CD56/NCAM1 using anti-CD56/NCAM1 antibody (A00184-4).
CD56/NCAM1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-CD56/NCAM1 Antibody (A00184-4) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of 293T cells using anti-CD56/NCAM1 antibody (A00184-4).
Overlay histogram showing 293T cells stained with A00184-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD56/NCAM1 Antibody (A00184-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Flow Cytometry analysis of U2OS cells using anti-CD56/NCAM1 antibody (A00184-4).
Overlay histogram showing U2OS cells stained with A00184-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD56/NCAM1 Antibody (A00184-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of CD56/NCAM1 using anti-CD56/NCAM1 antibody (A00184-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: Mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD56/NCAM1 antigen affinity purified polyclonal antibody (A00184-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD56/NCAM1 at approximately 120-150 kDa. The expected band size for CD56/NCAM1 is at 95 kDa.
IF analysis of CD56/NCAM1 using anti-CD56/NCAM1 antibody (A00184-4).
CD56/NCAM1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-CD56/NCAM1 Antibody (A00184-4) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of 293T cells using anti-CD56/NCAM1 antibody (A00184-4).
Overlay histogram showing 293T cells stained with A00184-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD56/NCAM1 Antibody (A00184-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Flow Cytometry analysis of U2OS cells using anti-CD56/NCAM1 antibody (A00184-4).
Overlay histogram showing U2OS cells stained with A00184-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD56/NCAM1 Antibody (A00184-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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