Western blot (WB): | 1:1000-5000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:20 |
Flow Cytometry (FCM): | 1:20 |
Figure 1. Western blot analysis of anti-Alpha Tubulin/TUBA1B antibody (BM3885). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Alpha Tubulin/TUBA1B antigen affinity purified monoclonal antibody (BM3885) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alpha Tubulin/TUBA1B at approximately 55 kDa. The expected band size for Alpha Tubulin/TUBA1B is at 50 kDa.
Immunohistochemical analysis of paraffin-embedded human bladder, using alpha Tubulin Antibody.