Mouse CXCL16 ELISA Kit

96T/2480

C X C motif chemokine 16; CXCL16; CXCLG16; SCYB16; Small inducible cytokine B16; SR PSOX; SRPSOX; Transmembrane chemokine CXCL16

Expression system for standard: E.coli; Immunogen sequence: N27-P114

There is no detectable cross-reactivity with other relevant proteins.

15.6pg/ml-1000pg/ml

CXCL16

C-X-C motif chemokine 16

Q8BSU2  

Mouse CXCL16 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0742)

Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells. CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha.2 The gene for human CXCL16 is located on chromosome 17p13. The standard product used in this kit is recombinant mouse CXCL16, consisting of 88 amino acids with the molecular mass of 9.9KDa.

Catalog number	Description	Quantity
EK0742-CAP	96-well plate precoated with anti- mouse CXCL16 antibody	1
EK0742-ST	Lyophilized recombinant mouse CXCL16 standard	10ng/tube×2
EK0742-DA	Biotinylated anti- mouse CXCL16 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	32	238	389	29	242	392
Standard deviation	1.28	10.71	27.61	1.24	12.34	29.79
CV(%)	4%	4.5%	7.1%	4.3%	5.1%	7.6%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.013	0.092	0.180	0.340	0.642	1.107	1.836	2.559

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h