产品名称
Human Beta-2 Microglobulin/B2M ELISA Kit
指标别名
B2M; Beta-2-microglobulin; HDCMA22P
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
beta-2-microglobulin
文献引用格式
Human Beta-2 Microglobulin/B2M ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1691)
背景资料
Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1691-CAP | 96-well plate precoated with anti- Human B2M antibody | 1 |
EK1691-ST | Lyophilized recombinant Human B2M standard | 10ng/tubex2 |
EK1691-DA | Biotinylated anti- Human B2M antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 293 | 1070 | 5097 | 310 | 1103 | 4757 |
Standard deviation | 21.97 | 62.06 | 219.17 | 26.97 | 69.48 | 285.42 |
CV(%) | 7.5 | 5.8 | 4.3 | 8.7 | 6.3 | 6 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.049 | 0.113 | 0.184 | 0.225 | 0.523 | 0.766 | 1.328 | 2.253 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, Urine and Human Milk