Human MDC/CCL22 Quick ELISA Kit

96T/3580

A-152E5.1; ABCD-1; DC/B-CK; MDC; SCYA22; STCP-1; CCL22

Expression system for standard: E.coli; Immunogen sequence: G25-Q93

There is no detectable cross-reactivity with other relevant proteins.

31.2pg/ml-2000pg/ml

CCL22

chemokine (C-C motif) ligand 22

O00626  

Human MDC/CCL22 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0446)

Macrophage-derived chemokine(MDC), also called Chemokine, cc motif, ligand 22(CCL22) or Small inducible cytokine subfamily A, member 22(SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine(TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

15分钟

Catalog number	Description	Quantity
FEK0446-CAP	Anti-Pre-coated 96-well Strip Microplate	1
FEK0446-ST	Human MDC standard	10ng/tube×2
FEK0446-A	Human MDC Antibody A	3ml
FEK0446-B	Human MDC Antibody B	3ml
AR1106-1	Sample diluent buffer	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	TMB color developing agent	10ml
AR1105	Stop solution	10ml
PLA-SEA	Adhesive cover	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	97	212	842	87	210	866
Standard deviation	7.37	9.11	55.57	7.91	12.6	64.08
CV(%)	7.6%	4.3%	6.6%	9.1%	6%	7.4%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.101	0.162	0.207	0.301	0.468	0.791	1.345	2.101

Store at 4℃ for 6 months.

ELISA

human

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)