产品名称
Mouse CXCL16 Quick ELISA Kit
指标别名
C X C motif chemokine 16; CXCL16; CXCLG16; SCYB16; Small inducible cytokine B16; SR PSOX; SRPSOX; Transmembrane chemokine CXCL16
免疫原
Expression system for standard: E.coli; Immunogen sequence: N27-P114
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
chemokine (C-X-C motif) ligand 16
文献引用格式
Mouse CXCL16 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0742)
背景资料
Chemokine(C-X-C motif) ligand 16(CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines(with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T(NKT) cells. CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha.2 The gene for human CXCL16 is located on chromosome 17p13. The standard product used in this kit is recombinant mouse CXCL16, consisting of 88 amino acids with the molecular mass of 9.9KDa.
试剂盒内容
Catalog number | Description | Quantity |
FEK0663-CAP | 96-well plate precoated with anti-Mouse CXCL16 antibody | 1 |
FEK0663-ST | lyophilized recombinant Mouse CXCL16 standard | 10ng/tube×2 |
FEK0663-A | Mouse CXCL16 Antibody A | 3ml |
FEK0742-B | Mouse CXCL16 Antibody B | 3ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 40 | 179 | 538 | 40 | 171 | 574 |
Standard deviation | 1.92 | 9.48 | 36.58 | 2.6 | 9.57 | 48.79 |
CV(%) | 4.8% | 5.3% | 6.8% | 6.5% | 5.6% | 8.5% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
O.D. | 0.016 | 0.187 | 0.329 | 0.600 | 1.058 | 1.774 | 2.276 | 2.405 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)