Anti-IL10 Antibody (Clone#AFIE-9)

Rat monoclonal antibody

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筛选器： All WB IHC

M00021-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of IL-10 using anti-IL-10 antibody (M00021-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse IL-10 protein 15ng,
Lane 2: recombinant mouse IL-10 protein 10ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rat anti-IL-10 antigen affinity purified monoclonal antibody (Catalog # M00021-1) at 0.5 μg/mL overnight at 4～C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
all(3)
IHC analysis of IL-10 using anti-IL-10 antibody (M00021-1).
IL-10 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml rat anti-IL-10 Antibody (M00021-1) overnight at 4～C. Biotinylated goat anti-rat IgG was used as secondary antibody and incubated for 30 minutes at 37～C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(3)
IHC analysis of IL-10 using anti-IL-10 antibody (M00021-1).
IL-10 was detected in paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml rat anti-IL-10 Antibody (M00021-1) overnight at 4～C. Biotinylated goat anti-rat IgG was used as secondary antibody and incubated for 30 minutes at 37～C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(3)

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产品简介>

产品名称

Anti-IL10 Antibody (Clone#AFIE-9)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

产品类型

Monoclonal

检验物种

mouse

应用范围

WB, IHC

基因名称

Il10

克隆号

AFIE-9

宿主

Rat

抗体亚型

IgG1, κ

免疫原

Recombinant mouse IL-10

计算分子量

21 kDa

成分

200 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

200 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Uniprot ID

P18893

文献引用格式

IL10 Antibody (Clone#AFIE-9) (Boster Biological Technology, Wuhan, China. Catalog#M00021-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

interleukin 10

蛋白名全称

Interleukin-10

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实验步骤

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