Anti-CD56/NCAM1 Antibody (Clone#OTI1G4)

Mouse monoclonal antibody

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筛选器： All WB IHC

M00184-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NCAM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NCAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCAM1 (Cat# M00184-3).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00184-3)
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产品简介>

产品名称

Anti-CD56/NCAM1 Antibody (Clone#OTI1G4)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD56; MSK39; N CAM 1; NCAM; NCAM 1; NCAM1; NCAM1/CD56

产品类型

Monoclonal

检验物种

human, mouse, rat, dog, monkey

应用范围

WB, IHC

基因名称

NCAM1

克隆号

OTI1G4

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 20-718 of human NCAM1 (NP_851996) produced in HEK293T cells.

计算分子量

95 kDa

实际分子量

94.4 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

NCAM is a membrane-bound glycoprotein that plays a role in cell-cell and cell-matrix adhesion through both its homophilic and heterophilic binding activity. The neural cell adhesion molecule appears on early embryonic cells and is important in the formation of cell collectives and their boundaries at sites of morphogenesis. Later in development it is found on various differentiated tissues and is a major CAM mediating adhesion among neurons and between neurons and muscle. NCAM gene is mapped to 11q23. The neural cell adhesion molecule (NCAM) can influence a number of diverse intercellular events, including junctional communication, the association of axons with pathways and targets, and signals that alter levels of neurotransmitter enzymes.

Uniprot ID

P13591

文献引用格式

CD56/NCAM1 Antibody (Clone#OTI1G4) (Boster Biological Technology, Wuhan, China. Catalog#M00184-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

neural cell adhesion molecule 1

蛋白名全称

Neural cell adhesion molecule 1

实验方案>

实验步骤

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