Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:20 |
Western blot analysis of anti-CCR7 antibody (BM4218). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCR7 antigen affinity purified monoclonal antibody (BM4218) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CCR7 at approximately 43 kDa. The expected band size for CCR7 is at 43 kDa.
Immunohistochemical analysis of paraffin-embedded human spleen, using CCR7 Antibody.
Immunofluorescent analysis of Hela cells, using CCR7 Antibody.
Western blot analysis of anti-CCR7 antibody (BM4218). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCR7 antigen affinity purified monoclonal antibody (BM4218) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CCR7 at approximately 43 kDa. The expected band size for CCR7 is at 43 kDa.
Immunohistochemical analysis of paraffin-embedded human spleen, using CCR7 Antibody.
Immunofluorescent analysis of Hela cells, using CCR7 Antibody.