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荧光二抗是一种经过荧光标记的第二抗体, 然后荧光二抗再与一抗结合。荧光二抗是一种免疫球蛋白,它的结构和普通抗体相似,具有两条重链和两条轻链,在重链和轻链的可变区可以特异性地识别一抗的Fc段(可结晶片段)。其独特之处在于它携带了荧光基团,这个荧光基团能够在特定波长的激发光下发出荧光,从而实现对目标抗原的可视化检测。博士德公司采用多种不同颜色的荧光标记,如FITC  CY3  Dylight 488、Dylight 594 、Dylight 647 、Dylight 550等,其荧光强度高、光稳定性好、荧光更稳定,持久,可用于免疫荧光技术、流式细胞术、Western blotting 的荧光检测。



荧光偶联物
最大激发/发射光
货号
名称
描述


FITC


Amax=495nm; Emax=525nm

BA1105
FITC Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
FITC-羊抗兔IgG
BA1101
FITC Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)
FITC-羊抗小鼠IgG
BM2012
FITC Conjugated AffiniPure Mouse Anti-Rabbit IgG (H+L)
FITC-小鼠抗兔IgG
BA1108
FITC Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L)
FITC-兔抗大鼠IgG
BA1110
FITC Conjugated AffiniPure Rabbit Anti-Goat IgG (H+L)
FITC-兔抗山羊IgG
BA1116
FITC Conjugated AffiniPure Goat Anti-Human IgM μ Chain
FITC-羊抗人IgM
BA1114
FITC Conjugated AffiniPure Rabbit Anti-Human IgG (H+L)
FITC-兔抗人IgG
BA1112
FITC Conjugated AffiniPure Goat Anti-Human IgA
FITC-羊抗人IgA
BA1120
FITC Conjugated Protein A
FITC-Protein A
BA1121
FITC Conjugated Protein G
FITC-Protein G
BA1125
FITC Conjugated Avidin
FITC-Avidin


DyLight 488 (绿光推荐)


Amax=493nm;Emax=518nm

BA1127
DyLight 488 Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
DyLight 488-羊抗兔IgG
BA1126
DyLight 488 Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)
DyLight 488-羊抗小鼠IgG
BA1145
DyLight 488 Conjugated AffiniPure Donkey Anti-Mouse IgG (H+L)
DyLight 488 驴抗小鼠 IgG
BA1146
DyLight 488 Conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L)
DyLight 488 驴抗兔 IgG
BA1129
DyLight 488 Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L)
DyLight 488-兔抗大鼠IgG
BA1124
DyLight 488 Conjugated AffiniPure Rabbit Anti-Goat IgG (H+L)
DyLight 488-兔抗山羊IgG
BA1045
DyLight 488 Conjugated AffiniPure Rabbit IgG
DyLight 488 标记兔 IgG
BA1128
DyLight 488 Conjugated Avidin
DyLight 488 标记亲和素


CY3


Amax=554nm; Emax=568nm

BA1032
CY3 Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
CY3-羊抗兔IgG
BA1031
CY3 Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)
CY3-羊抗小鼠IgG
BA1035
CY3 Conjugated AffiniPure Rabbit Anti-Human IgG (H+L)
CY3-兔抗人IgG
BA1034
CY3 Conjugated AffiniPure Rabbit Anti-Goat IgG (H+L)
CY3-兔抗羊IgG
BA1033
CY3 Conjugated Protein A
CY3-Protein A
BA1037
CY3 Conjugated Avidin
CY3-Avidin


DyLight 550 (橙色推荐)


Amax=562nm; Emax=576nm

BA1133
DyLight 550 Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)
DyLight 550-羊抗小鼠(H+L)
BA1135
DyLight 550 Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
DyLight 550-羊抗兔(H+L)
BA1149
Dylight 550 Conjugated AffiniPure Donkey Anti-Mouse IgG (H+L)
DyLight 550-驴抗小鼠 IgG(H+L)
BA1144
Dylight 550 Conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L)
DyLight 550-驴抗兔 IgG(H+L)
BA1134
DyLight 550 Conjugated Avidin
DyLight 550 标记亲和素


TRITC


Amax=550nm; Emax=620nm

BA1090
TRITC Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
TRITC-羊抗兔IgG
BA1089
TRITC Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)
TRITC-羊抗小鼠IgG
BA1091
TRITC Conjugated AffiniPure Rabbit Anti-Goat IgG (H+L)
TRITC-兔抗山羊IgG
BA1094
TRITC Conjugated AffiniPure Mouse Anti-Human IgG (H+L)
TRITC-小鼠抗人IgG
BA1092
TRITC Conjugated AffiniPure Rabbit Anti-Human IgG (H+L)
TRITC-兔抗人IgG
BA1093
TRITC Conjugated Avidin
TRITC 标记亲和素


DyLight 594 (红光推荐)


Amax=593nm; Emax=618nm

BA1142
DyLight 594 Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
DyLight 594-山羊抗兔(H+L)
BA1141
DyLight 594 Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)
DyLight 594-山羊抗小鼠(H+L)
BA1148
Dylight 594 Conjugated AffiniPure Donkey Anti-Mouse IgG (H+L)
DyLight 594-驴抗小鼠 IgG(H+L)
BA1147
Dylight 594 Conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L)
DyLight 594-驴抗兔 IgG(H+L)
BA1143
DyLight 594 Conjugated Avidin
DyLight 594 标记亲和素

DyLight 647

Amax=652nm; Emax=664nm

BA1150
DyLight 647 Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
DyLight 647-羊抗兔IgG
BA1151
DyLight 647 Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)
DyLight 647-羊抗小鼠IgG




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