Anti-TLR4 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB ELISA

A00017-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of TLR4 using anti-TLR4 antibody (A00017-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TLR4 antigen affinity purified polyclonal antibody (A00017-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TLR4 at approximately 96 kDa. The expected band size for TLR4 is at 96 kDa.
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产品简介>

产品名称

Anti-TLR4 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ARMD10; CD284; hToll; TLR4; TOLL; toll like receptor 4

产品类型

Polyclonal

检验物种

mouse

应用范围

WB, ELISA

基因名称

TLR4

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse Tlr4 recombinant protein (Position: N26-E827).

计算分子量

96 kDa

实际分子量

96 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Toll-like receptor 4 is a protein that in humans is encoded by the TLR4 gene. This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls.

Uniprot ID

Q9QUK6

文献引用格式

TLR4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00017-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

toll-like receptor 4

蛋白名全称

Toll-like receptor 4

实验方案>

实验步骤

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