Anti-Caspase 8/CASP8 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM

A00042

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Caspase 8/CASP8 using anti-Caspase 8/CASP8 antibody (A00042). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: HEPG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 8/CASP8 antigen affinity purified polyclonal antibody (A00042) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 8/CASP8 at approximately 55 kDa. The expected band size for Caspase 8/CASP8 is at 55 kDa.
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IHC analysis of Caspase 8/CASP8 using anti-Caspase 8/CASP8 antibody (A00042).
Caspase 8/CASP8 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Caspase 8/CASP8 Antibody (A00042) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Caspase 8/CASP8 using anti-Caspase 8/CASP8 antibody (A00042).
Caspase 8/CASP8 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Caspase 8/CASP8 Antibody (A00042) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Caspase 8/CASP8 using anti-Caspase 8/CASP8 antibody (A00042).
Caspase 8/CASP8 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Caspase 8/CASP8 Antibody (A00042) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Caspase 8/CASP8 using anti-Caspase 8/CASP8 antibody (A00042).
Caspase 8/CASP8 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Caspase 8/CASP8 Antibody (A00042) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Caspase 8/CASP8 using anti-Caspase 8/CASP8 antibody (A00042).
Caspase 8/CASP8 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Caspase 8/CASP8 Antibody (A00042) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(8)
Flow Cytometry analysis of PC-3 cells using anti-CASP8 antibody (A00042).Overlay histogram showing PC-3 cells stained with A00042 (Blue line).. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Flow Cytometry analysis of Hela cells using anti-CASP8 antibody (A00042).Overlay histogram showing Hela cells stained with A00042 (Blue line).. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品简介>

产品名称

Anti-Caspase 8/CASP8 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apoptotic protease Mch 5; Casp 8; CASP8; CASP-8; Caspase 8; caspase8; FADD like ICE; FLICE; ICE like apoptotic protease 5; MCH5

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM

基因名称

CASP8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase 8, different from the related mouse and rat sequences by seven amino acids.

计算分子量

55 kDa

实际分子量

55 kDa

成分

500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. In addtion, this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Uniprot ID

Q14790

文献引用格式

Caspase 8/CASP8 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00042)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Caspase 8, apoptosis-related cysteine peptidase

蛋白名全称

Caspase 8, apoptosis-related cysteine peptidase

实验方案>

实验步骤

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