Anti-NOX2/CYBB Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB ELISA

A00328

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of NOX2/CYBB using anti-NOX2/CYBB antibody (A00328). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOX2/CYBB antigen affinity purified polyclonal antibody (A00328) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NOX2/CYBB at approximately 65 kDa. The expected band size for NOX2/CYBB is at 65 kDa.
all(2)
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-NOX2/CYBB Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CYBB; Cytochrome b 245 heavy chain; NOX2; p91 PHOX

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ELISA

基因名称

CYBB

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human NOX2/gp91phox recombinant protein (Position: F416-D500).

计算分子量

65 kDa

实际分子量

65 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

NOX2(NADPH OXIDASE 2), also called CYBB(CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein. NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling).

Uniprot ID

P04839

RRID

AB_3081059

文献引用格式

NOX2/CYBB Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00328)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

引用文献(0)>

清除