Anti-iNOS/NOS2 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB FCM ELISA

A00368-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti- NOS2 antibody (A00368-4). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: mouse RAW264.7(-LPS) whole cell lysates,
Lane 2: mouse RAW264.7(+LPS) whole cell lysates.
Use rabbit anti- NOS2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for NOS2 at approximately 130KD. The expected band size for NOS2 is at 130KD.
all(3)
Flow Cytometry analysis of Hepa1-6 cells using anti-iNOS/NOS2 antibody (A00368-4).
Overlay histogram showing Hepa1-6 cells stained with A00368-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-iNOS/NOS2 Antibody (A00368-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
all(3)
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-iNOS/NOS2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

HEP NOS; Hepatocyte NOS; Inducible NO synthase; Inducible NOS; INOS; NOS; NOS type II; NOS2; NOS2A

产品类型

Polyclonal

检验物种

mouse

应用范围

WB, FCM, ELISA

基因名称

NOS2

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse iNOS/Nos2 recombinant protein (Position: I40-L1099).

计算分子量

130 kDa

实际分子量

130 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Nitric oxide synthase, inducible is an enzyme that in humans is encoded by the NOS2 gene. Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter; it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. Three different NOS isoforms have been identified which fall into two distinct types, constitutive and inducible. The inducible NOS (iNOS) isoform is expressed in a variety of cell types and tissues in response to inflammatory agents and cytokines. The human iNOS (NOS2) gene is approximately 37 kb in length and consists of 26 exons and 25 introns. NOS2-derived NO is a prerequisite for cytokine signaling and function in innate immunity.

Uniprot ID

P29477

文献引用格式

iNOS/NOS2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00368-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

nitric oxide synthase 2, inducible

蛋白名全称

Nitric oxide synthase, inducible

实验方案>

实验步骤

引用文献(0)>

清除