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Anti-ANGPT2 Antibody

Rabbit polyclonal antibody

说明书

筛选器: All WB ICC/IF FCM IHC ELISA

A00370-2

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Figure 1. Western blot analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human SW620 whole cell lysates,
    Lane 2: human A549 whole cell lysates,
    Lane 3: human K562 whole cell lysates,
    Lane 4: human Raji whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: mouse spleen tissue lysates,
    Lane 7: mouse SP2/0 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ANGPT2 antigen affinity purified polyclonal antibody (A00370-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ANGPT2 at approximately 70 kDa,51 kDa. The expected band size for ANGPT2 is at 57 kDa、51 kDa.

    all(8)
  • Figure 2. IF analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2).
    ANGPT2 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-ANGPT2 Antibody (A00370-2) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(8)
  • Figure 3. Flow Cytometry analysis of Hela cells using anti-ANGPT2 antibody (A00370-2).
    Overlay histogram showing Hela cells stained with A00370-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANGPT2 Antibody (A00370-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

    all(8)
  • Figure 4. Flow Cytometry analysis of U937 cells using anti-ANGPT2 antibody (A00370-2).
    Overlay histogram showing U937 cells stained with A00370-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANGPT2 Antibody (A00370-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

    all(8)
  • Figure 5. IHC analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2).
    ANGPT2 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ANGPT2 Antibody (A00370-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

    all(8)
  • Figure 6. IHC analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2).
    ANGPT2 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ANGPT2 Antibody (A00370-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

    all(8)
  • Figure 7. IHC analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2).
    ANGPT2 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ANGPT2 Antibody (A00370-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

    all(8)

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产品名称
Anti-ANGPT2 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
AGPT2; ANG 2; ANG2; angiopoietin 2; Angiopoietin-2; ANGPT2; PDGFAB
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, ICC/IF, FCM, IHC, ELISA
基因名称
ANGPT2
宿主
Rabbit
抗体亚型
IgG
免疫原
E. coli-derived human Angiopoietin-2/ANGPT2 recombinant protein (Position: N357-K404). Human ANGPT2 shares 83% amino acid (aa) sequence identity with both mouse and rat ANGPT2.
计算分子量
57 kDa、51 kDa
实际分子量
70 kDa,51 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
ANGPT2,also known as ANG2 or Angiopoietin 2,is a protein that in humans is encoded by the ANGPT2 gene. It is mapped to 8p23.1. ANGPT2 is a naturally occurring antagonist of ANG1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. ANGPT2 was significantly increased in plasma and alveolar edema fluid in adults with acute lung injury compared to controls or patients with hydrostatic pulmonary edema,tracheal. ANGPT2 was also significantly increased in neonates with respiratory distress syndrome who developed bronchopulmonary edema. It is also a mediator of epithelial necrosis with an important role in hyperoxic acute lung injury and pulmonary edema.
Uniprot ID
O15123  
RRID
文献引用格式
ANGPT2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00370-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
angiopoietin 2
蛋白名全称
Angiopoietin-2
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
Flow Cytometry (Fixed):1:50-200
Enzyme linked immunosorbent assay (ELISA):1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-ANGPT2 Antibody

    筛选器: All WB ICC/IF FCM IHC ELISA

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