Anti-KEAP1 Antibody

筛选器： All WB ICC/IF FCM ELISA

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  Western blot analysis of KEAP1 using anti-KEAP1 antibody (A00514-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: Jurkat whole cell lysates,
  Lane 3: 293T whole cell lysates,
  Lane 4: HepG2 whole cell lysates,
  Lane 5: C6 whole cell lysates,
  Lane 6: Neuro-2a whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-KEAP1 antigen affinity purified polyclonal antibody (A00514-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for KEAP1 at approximately 66-72 kDa. The expected band size for KEAP1 is at 70 kDa.

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  IF analysis of KEAP1 using anti-KEAP1 antibody (A00514-3).
  KEAP1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-KEAP1 Antibody (A00514-3) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of U251 cells using anti-KEAP1 antibody (A00514-3).
  Overlay histogram showing U251 cells stained with A00514-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KEAP1 Antibody (A00514-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of KEAP1 using anti-KEAP1 antibody (A00514-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: Jurkat whole cell lysates,
  Lane 3: 293T whole cell lysates,
  Lane 4: HepG2 whole cell lysates,
  Lane 5: C6 whole cell lysates,
  Lane 6: Neuro-2a whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-KEAP1 antigen affinity purified polyclonal antibody (A00514-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for KEAP1 at approximately 66-72 kDa. The expected band size for KEAP1 is at 70 kDa.

• 

  IF analysis of KEAP1 using anti-KEAP1 antibody (A00514-3).
  KEAP1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-KEAP1 Antibody (A00514-3) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of U251 cells using anti-KEAP1 antibody (A00514-3).
  Overlay histogram showing U251 cells stained with A00514-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KEAP1 Antibody (A00514-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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