Anti-NKG2D/KLRK1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

A00661-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of NKG2D/KLRK1 using anti-NKG2D/KLRK1 antibody (A00661-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lymph tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NKG2D/KLRK1 antigen affinity purified polyclonal antibody (A00661-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NKG2D/KLRK1 at approximately 25 kDa. The expected band size for NKG2D/KLRK1 is at 25 kDa .
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产品简介>

产品名称

Anti-NKG2D/KLRK1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD314; D12S2489E; FLJ17759; FLJ75772; KLR; KLRK1; NK cell receptor D; NKG2 D; NKG2 D activating NK receptor; NKG2D

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

基因名称

KLRK1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human NKG2D, which shares 76.7% amino acid (aa) sequence identity with both mouse and rat NKG2D.

计算分子量

25 kDa

实际分子量

25 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

NKG2D is encoded by KLRK1 gene which is located in the NK-gene complex (NKC) situated on and chromosome 12 in humans. Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster.

Uniprot ID

P26718

文献引用格式

NKG2D/KLRK1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00661-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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