Anti-TMEM16A/DOG1/ANO1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM

A00713

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TMEM16A/DOG1/ANO1 using anti-TMEM16A/DOG1/ANO1 antibody (A00713). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human PANC-1 whole cell lysates,
Lane 5: human SK-OV-3 whole cell lysates,
Lane 6: human SGC-7901 whole cell lysates,
Lane 7: human COLO-320 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TMEM16A/DOG1/ANO1 antigen affinity purified polyclonal antibody (A00713) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TMEM16A/DOG1/ANO1 at approximately 130 kDa. The expected band size for TMEM16A/DOG1/ANO1 is at 114 kDa.
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IHC analysis of TMEM16A/DOG1/ANO1 using anti-TMEM16A/DOG1/ANO1 antibody (A00713).
TMEM16A/DOG1/ANO1 was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TMEM16A/DOG1/ANO1 Antibody (A00713) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of TMEM16A/DOG1/ANO1 using anti-TMEM16A/DOG1/ANO1 antibody (A00713).
TMEM16A/DOG1/ANO1 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TMEM16A/DOG1/ANO1 Antibody (A00713) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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Flow Cytometry analysis of A431 cells using anti-TMEM16A/DOG1/ANO1 antibody (A00713).
Overlay histogram showing A431 cells stained with A00713 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM16A/DOG1/ANO1 Antibody (A00713) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介>

产品名称

Anti-TMEM16A/DOG1/ANO1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ANO1; Anoctamin 1; DOG1; ORAOV2; TAOS2; TMEM16A; TMEM16A/DOG1; Transmembrane protein 16A

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, FCM

基因名称

ANO1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human TMEM16A, which shares 83.8% amino acid (aa) sequence identity with both mouse and rat TMEM16A.

计算分子量

114 kDa

实际分子量

130 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Anoctamin-1 (ANO1), also known as oral cancer overexpressed 2 (ORAOV2) or tumor-amplified and overexpressed sequence 2(TMEM16A), is a protein that in humans is encoded by the ANO1 gene. This gene belongs to a family of membrane proteins containing 8 transmembrane segments, and it is mapped to 11q13.3. ANO1 is a candidate calcium-activated chloride channel that mediates receptor-activated chloride currents in diverse physiologic processes, and it is thought to be responsible for a voltage-sensitive calcium-activated chloride current. Its overexpression was reported in esophageal squamous cell carcinoma and breast cancer progression Crofelemer, an antidiarrhoeal, inhibits this channel. ANO1 has eight transmembrane domains, its pore is large and non-selective, allowing other negatively charged species to permeate.

Uniprot ID

Q5XXA6

RRID

AB_3081218

文献引用格式

TMEM16A/DOG1/ANO1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00713)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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