Anti-ESR2 Antibody

Rabbit polyclonal antibody

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A00786-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of ESR2 using anti-ESR2 antibody (A00786-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: PC-3 whole cell lysates,
Lane 2: MCF-7 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse testis tissue lysates,
Lane 7: NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ESR2 antigen affinity purified polyclonal antibody (A00786-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ESR2 at approximately 59 kDa. The expected band size for ESR2 is at 59 kDa.
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产品名称

Anti-ESR2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ER BETA; Erb; ESR BETA; ESR2; ESRB; ESTRB; estrogen receptor 2 (ER beta); Estrogen receptor beta; Estrogen Receptor β; NR3A2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

ESR2

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human ESR2 recombinant protein (Position: F289-R501). Human ESR2 shares 93.4% and 93.9% amino acid (aa) sequence identity with mouse and rat ESR2, respectively.

计算分子量

59 kDa

实际分子量

59 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.

Uniprot ID

Q92731

文献引用格式

ESR2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00786-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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