Western blot analysis of GSK3B using anti-GSK3B antibody (A00791-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GSK3B antigen affinity purified polyclonal antibody (A00791-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GSK3B at approximately 47 kDa. The expected band size for GSK3B is at 47 kDa.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of GSK3B using anti-GSK3B antibody (A00791-3) and anti-Beta Tubulin antibody (M01857-3).
GSK3B was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of GSK3B using anti-GSK3B antibody (A00791-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GSK3B antigen affinity purified polyclonal antibody (A00791-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GSK3B at approximately 47 kDa. The expected band size for GSK3B is at 47 kDa.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of GSK3B using anti-GSK3B antibody (A00791-3) and anti-Beta Tubulin antibody (M01857-3).
GSK3B was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
Western blot analysis of GSK3B using anti-GSK3B antibody (A00791-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GSK3B antigen affinity purified polyclonal antibody (A00791-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GSK3B at approximately 47 kDa. The expected band size for GSK3B is at 47 kDa.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3) .
GSK3B was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of GSK3B using anti-GSK3B antibody (A00791-3) and anti-Beta Tubulin antibody (M01857-3).
GSK3B was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-GSK3B Antibody (A00791-3) at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
联系我们027-67845390
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390/1/2 技术支持：武汉丰网
© 1993-2025 Boster Biological Technology co.Itd E-mail:boster@boster.com
鄂ICP备05005548号-2
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
