Anti-GCK Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF ELISA

A00884-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of GCK using anti-GCK antibody (A00884-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GCK antigA03957-Aen affinity purified polyclonal antibody (A00884-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GCK at approximately 52 kDa. The expected band size for GCK is at 52 kDa.
all(3)
IF analysis of GCK using anti-GCK antibody (A00884-1).
GCK was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-GCK Antibody (A00884-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(3)
all(3)

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产品简介>

产品名称

Anti-GCK Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

GCK; GK; GLK; Glucokinase; glucokinase (hexokinase 4); Hexokinase 4; Hexokinase D; Hexokinase type IV; HHF3; HK IV; HK4; HKIV; HXKP; MODY2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, ELISA

基因名称

GCK

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human Glucokinase recombinant protein (Position: Y234-T431).

计算分子量

52 kDa

实际分子量

52 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Glucokinase(GCK) is an enzyme that facilitates phosphorylation of glucose to glucose-6-phosphate. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

Uniprot ID

P35557

文献引用格式

GCK Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00884-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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