Anti-IL12A Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A00918-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of IL12A using anti-IL12A antibody (A00918-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL12A antigen affinity purified polyclonal antibody (A00918-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL12A at approximately 25 kDa. The expected band size for IL12A is at 25 kDa.
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IHC analysis of IL12A using anti-IL12A antibody (A00918-1).
IL12A was detected in a paraffin-embedded section of mouse small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-IL12A Antibody (A00918-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of IL12A using anti-IL12A antibody (A00918-1).
IL12A was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-IL12A Antibody (A00918-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
all(4)

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产品简介>

产品名称

Anti-IL12A Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CLMF; CLMF p35; IL 12 subunit p35; IL 12A; IL12; IL-12; IL12A; IL-12A; Interleukin 12 subunit alpha; NFSK; NKSF1; P35

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ELISA

基因名称

Il12a

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived rat IL12A recombinant protein (Position: R23-S215). Rat IL12A shares 59.5% and 87% amino acid (aa) sequence identity with human and mouse IL12A, respectively.

计算分子量

25 kDa

实际分子量

25 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Interleukin-12 subunit alpha (IL-12 p35) is a protein that in humans is encoded by the IL12A gene. This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.

Uniprot ID

Q9R103

RRID

AB_3081287

文献引用格式

IL12A Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00918-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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