Anti-CYP11A1 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB ELISA

A01071-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of CYP11A1 using anti-CYP11A1 antibody (A01071-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat ovary tissue lysates,
Lane 3: mouse ovary tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CYP11A1 antigen affinity purified polyclonal antibody (A01071-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CYP11A1 at approximately 50-60 kDa. The expected band size for CYP11A1 is at 60 kDa.
all(2)
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CYP11A1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cholesterol desmolase; CYP11A; CYP11A1; CYPXIA1; Cytochrome P450 11A1; Cytochrome P450(scc); P450SCC

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ELISA

基因名称

CYP11A1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CYP11A1 recombinant protein (Position: H72-Q461).

计算分子量

60 kDa

实际分子量

50-60 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CYP11A1 (Cytochrome p450, family 11, subfamily A, polypeptide 1), commonly referred to as P450scc, is a mitochondrial enzyme associated with the conversion of cholesterol to pregnenolone. CYP11A1 is a member of the cytochrome P450 superfamily of enzymes. The CYP11A1 gene is mapped on 15q24.1. Expression of the CYP11A1 gene may play a role in skin physiology and pathology and that cutaneous proopiomelanocortin activity may be autoregulated by a feedback mechanism involving glucocorticoids synthesized locally. Using in vitro studies, CYP11A1 catalyzed the side-chain cleavage of 7-dehydrocholesterol to form 7-dehydropregnenolone. In addition, CYP11A1 catalyzed the metabolism of biologically inert vitamin D3, which is formed from 7-dehydrocholesterol, to form 2 hydroxylated products, 20-hydroxyvitamin D3 and 20, 22-dihydroxyvitamin D3. Mutations in the CYP11A1 gene cause congenital adrenal insufficiency with partial or complete 46, XY sex reversal.

Uniprot ID

P05108

文献引用格式

CYP11A1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01071-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cytochrome P450 family 11 subfamily A member 1

蛋白名全称

Cholesterol side-chain cleavage enzyme, mitochondrial

实验方案>

实验步骤

引用文献(0)>

清除