Western blot (WB): | 1:500-2000 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
Western blot analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: rat stomach tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse stomach tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ALDH3A1 antigen affinity purified polyclonal antibody (A01121-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALDH3A1 at approximately 55 kDa. The expected band size for ALDH3A1 is at 50 kDa.
IF analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-3).
ALDH3A1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-ALDH3A1 Antibody (A01121-3) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Western blot analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: rat stomach tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse stomach tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ALDH3A1 antigen affinity purified polyclonal antibody (A01121-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALDH3A1 at approximately 55 kDa. The expected band size for ALDH3A1 is at 50 kDa.
IF analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-3).
ALDH3A1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-ALDH3A1 Antibody (A01121-3) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).