Anti-JMJD3/KDM6B Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of JMJD3/KDM6B using anti-JMJD3/KDM6B antibody (A01309-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human K562 whole cell lysates,
  Lane 2: human A375 whole cell lysates,
  Lane 3: human HEK293 whole cell lysates,
  Lane 4: human A431 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-JMJD3/KDM6B antigen affinity purified polyclonal antibody (A01309-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for JMJD3/KDM6B at approximately 177 kDa. The expected band size for JMJD3/KDM6B is at 177 kDa.

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  Flow Cytometry analysis of K562 cells using anti-JMJD3/KDM6B antibody (A01309-1).
  Overlay histogram showing K562 cells stained with A01309-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JMJD3/KDM6B Antibody (A01309-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of Hepa1-6 cells using anti-JMJD3/KDM6B antibody (A01309-1).
  Overlay histogram showing Hepa1-6 cells stained with A01309-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JMJD3/KDM6B Antibody (A01309-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of JMJD3/KDM6B using anti-JMJD3/KDM6B antibody (A01309-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human K562 whole cell lysates,
  Lane 2: human A375 whole cell lysates,
  Lane 3: human HEK293 whole cell lysates,
  Lane 4: human A431 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-JMJD3/KDM6B antigen affinity purified polyclonal antibody (A01309-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for JMJD3/KDM6B at approximately 177 kDa. The expected band size for JMJD3/KDM6B is at 177 kDa.

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  Flow Cytometry analysis of K562 cells using anti-JMJD3/KDM6B antibody (A01309-1).
  Overlay histogram showing K562 cells stained with A01309-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JMJD3/KDM6B Antibody (A01309-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of Hepa1-6 cells using anti-JMJD3/KDM6B antibody (A01309-1).
  Overlay histogram showing Hepa1-6 cells stained with A01309-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JMJD3/KDM6B Antibody (A01309-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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