Anti-CD74 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

A01340-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CD74 using anti-CD74 antibody (A01340-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD74 antigen affinity purified polyclonal antibody (A01340-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD74 at approximately 34 kDa. The expected band size for CD74 is at 34 kDa.
all(3)
IHC analysis of CD74 using anti-CD74 antibody (A01340-3).
CD74 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD74 Antibody (A01340-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IHC analysis of CD74 using anti-CD74 antibody (A01340-3).
CD74 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD74 Antibody (A01340-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-CD74 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD74; DHLAG; HLADG; Ia GAMMA; Ii; p33

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

CD74

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human CD74, which shares 61.9% and 57.1% amino acid (aa) sequence identity with mouse and rat CD74, respectively.

计算分子量

34 kDa

实际分子量

34 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

HLA class II histocompatibility antigen gamma chain also known as HLA-DR antigens-associated invariant chain or CD74 (Cluster of Differentiation 74), is a protein that in humans is encoded by the CD74 gene. The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Uniprot ID

P04233

文献引用格式

CD74 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01340-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD74 molecule

蛋白名全称

HLA class II histocompatibility antigen gamma chain

实验方案>

实验步骤

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