Anti-LC3A/MAP1LC3A Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A01543-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of LC3A/MAP1LC3A using anti-LC3A/MAP1LC3A antibody (A01543-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LC3A/MAP1LC3A antigen affinity purified polyclonal antibody (A01543-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LC3A/MAP1LC3A at approximately 18 kDa. The expected band size for LC3A/MAP1LC3A is at 14 kDa.
all(4)
IHC analysis of LC3A/MAP1LC3A using anti-LC3A/MAP1LC3A antibody (A01543-1).
LC3A/MAP1LC3A was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LC3A/MAP1LC3A Antibody (A01543-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of LC3A/MAP1LC3A using anti-LC3A/MAP1LC3A antibody (A01543-1).
LC3A/MAP1LC3A was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LC3A/MAP1LC3A Antibody (A01543-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
all(4)

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产品简介>

产品名称

Anti-LC3A/MAP1LC3A Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

LC3; LC3A; MAP1A/MAP1B light chain 3 A; MAP1ALC3; MAP1BLC3; MAP1LC3A

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ELISA

基因名称

MAP1LC3A

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human MAP1LC3A recombinant protein (Position: M1-G120).

计算分子量

14 kDa

实际分子量

18 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Microtubule-associated proteins 1A/1B light chain 3A is a protein that in humans is encoded by the MAP1LC3A gene. MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.

Uniprot ID

Q9H492

文献引用格式

LC3A/MAP1LC3A Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01543-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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