Western blot (WB): | 1:500-2000 |
ELISA: | 1:100-1000 |
Western blot analysis of LAMP2 using anti-LAMP2 antibody (A01573-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: U-87MG whole cell lysates,
Lane 2: PC-3 whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: Jurkat whole cell lysates,
Lane 5: HepG2 whole cell lysates,
Lane 6: Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LAMP2 antigen affinity purified polyclonal antibody (A01573-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LAMP2 at approximately 100-120 kDa. The expected band size for LAMP2 is at 46 kDa.
Western blot analysis of LAMP2 using anti-LAMP2 antibody (A01573-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: U-87MG whole cell lysates,
Lane 2: PC-3 whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: Jurkat whole cell lysates,
Lane 5: HepG2 whole cell lysates,
Lane 6: Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LAMP2 antigen affinity purified polyclonal antibody (A01573-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LAMP2 at approximately 100-120 kDa. The expected band size for LAMP2 is at 46 kDa.