Western blot (WB): | 1:500-2000 |
ELISA: | 1:100-1000 |
Figure 1. Western blot analysis of TPH1 using anti-TPH1 antibody (A01626-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SGC-7901 whole cell lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TPH1 antigen affinity purified polyclonal antibody (A01626-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TPH1 at approximately 51 kDa. The expected band size for TPH1 is at 51 kDa.
Figure 1. Western blot analysis of TPH1 using anti-TPH1 antibody (A01626-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SGC-7901 whole cell lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TPH1 antigen affinity purified polyclonal antibody (A01626-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TPH1 at approximately 51 kDa. The expected band size for TPH1 is at 51 kDa.