Western blot analysis of NFAT5 using anti-NFAT5 antibody (A01815-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U2OS whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFAT5 antigA03957-Aen affinity purified polyclonal antibody (A01815-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NFAT5 at approximately 220 kDa. The expected band size for NFAT5 is at 166 kDa.
Western blot analysis of NFAT5 using anti-NFAT5 antibody (A01815-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFAT5 antigA03957-Aen affinity purified polyclonal antibody (A01815-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NFAT5 at approximately 220 kDa. The expected band size for NFAT5 is at 166 kDa.
IHC analysis of NFAT5 using anti-NFAT5 antibody (A01815-3).
NFAT5 was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was incubated with rabbit anti-NFAT5 Antibody (A01815-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of NFAT5 using anti-NFAT5 antibody (A01815-3).
NFAT5 was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was incubated with rabbit anti-NFAT5 Antibody (A01815-3) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) (Catalog # BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of NFAT5 using anti-NFAT5 antibody (A01815-3) and anti-Beta Tubulin antibody (M01857-3).
NFAT5 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-NFAT5 Antibody (A01815-3) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.
Flow Cytometry analysis of Hela cells using anti-NFAT5 antibody (A01815-3).
Overlay histogram showing Hela cells stained with A01815-3 (Blue line). To facilitate intrNFAT5llular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFAT5 Antibody (A01815-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of NFAT5 using anti-NFAT5 antibody (A01815-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U2OS whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFAT5 antigA03957-Aen affinity purified polyclonal antibody (A01815-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NFAT5 at approximately 220 kDa. The expected band size for NFAT5 is at 166 kDa.
Western blot analysis of NFAT5 using anti-NFAT5 antibody (A01815-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFAT5 antigA03957-Aen affinity purified polyclonal antibody (A01815-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NFAT5 at approximately 220 kDa. The expected band size for NFAT5 is at 166 kDa.
IHC analysis of NFAT5 using anti-NFAT5 antibody (A01815-3).
NFAT5 was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was incubated with rabbit anti-NFAT5 Antibody (A01815-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of NFAT5 using anti-NFAT5 antibody (A01815-3).
NFAT5 was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was incubated with rabbit anti-NFAT5 Antibody (A01815-3) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) (Catalog # BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of NFAT5 using anti-NFAT5 antibody (A01815-3) and anti-Beta Tubulin antibody (M01857-3).
NFAT5 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-NFAT5 Antibody (A01815-3) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.
Flow Cytometry analysis of Hela cells using anti-NFAT5 antibody (A01815-3).
Overlay histogram showing Hela cells stained with A01815-3 (Blue line). To facilitate intrNFAT5llular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFAT5 Antibody (A01815-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of NFAT5 using anti-NFAT5 antibody (A01815-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U2OS whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFAT5 antigA03957-Aen affinity purified polyclonal antibody (A01815-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NFAT5 at approximately 220 kDa. The expected band size for NFAT5 is at 166 kDa.
Western blot analysis of NFAT5 using anti-NFAT5 antibody (A01815-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFAT5 antigA03957-Aen affinity purified polyclonal antibody (A01815-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NFAT5 at approximately 220 kDa. The expected band size for NFAT5 is at 166 kDa.
IHC analysis of NFAT5 using anti-NFAT5 antibody (A01815-3).
NFAT5 was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was incubated with rabbit anti-NFAT5 Antibody (A01815-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of NFAT5 using anti-NFAT5 antibody (A01815-3).
NFAT5 was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was incubated with rabbit anti-NFAT5 Antibody (A01815-3) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) (Catalog # BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of NFAT5 using anti-NFAT5 antibody (A01815-3) and anti-Beta Tubulin antibody (M01857-3).
NFAT5 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-NFAT5 Antibody (A01815-3) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.
Flow Cytometry analysis of Hela cells using anti-NFAT5 antibody (A01815-3).
Overlay histogram showing Hela cells stained with A01815-3 (Blue line). To facilitate intrNFAT5llular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFAT5 Antibody (A01815-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
联系我们027-67845390
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390/1/2 技术支持：武汉丰网
© 1993-2025 Boster Biological Technology co.Itd E-mail:boster@boster.com
鄂ICP备05005548号-2
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
