Western blot (WB): | 1:500-2000 |
Western blot analysis of CCR1 using anti-CCR1 antibody (A01896-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCR1 antigen affinity purified polyclonal antibody (A01896-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CCR1 at approximately 41 kDa. The expected band size for CCR1 is at 41 kDa.
Western blot analysis of CCR1 using anti-CCR1 antibody (A01896-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCR1 antigen affinity purified polyclonal antibody (A01896-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CCR1 at approximately 41 kDa. The expected band size for CCR1 is at 41 kDa.