产品名称
Anti-CHRNA3 DyLight 488 Conjugated Antibody
指标别称
CHRNA3; LNCR2; NACHRA3; PAOD2
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human CHRNA3, which shares 97.4% and 94.7% amino acid (aa) sequence identity with mouse and rat CHRNA3, respectively.
激发/发射光谱
Amax=488nm; Emax=515-545nm
成分
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
纯化方式
Immunogen affinity purified.
保存条件
At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
背景资料
Neuronal acetylcholine receptor subunit alpha-3, also known as nAChRα3, is a protein that in humans is encoded by the CHRNA3 gene. This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.
文献引用格式
CHRNA3 DyLight 488 Conjugated Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01981-Dyl488)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
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