Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
Immunofluorescence (IF): | 1:50-400 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. Western blot analysis of anti-MRC1 antibody (A02285-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: human liver tissue lysates,
Lane 4: monkey lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MRC1 antigen affinity purified polyclonal antibody (A02285-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MRC1 at approximately 190-200 kDa. The expected band size for MRC1 is at 166 kDa.
Figure 2. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
MRC1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 3. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
MRC1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 4. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
MRC1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 5. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
MRC1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 6. IF analysis of CD206/MRC1 using anti-CD206/MRC1 antibody (A02285-2).
CD206/MRC1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD206/MRC1 Antibody (A02285-2) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. IF analysis of CD206/MRC1 using anti-CD206/MRC1 antibody (A02285-2).
CD206/MRC1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-CD206/MRC1 Antibody (A02285-2) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 1. Western blot analysis of anti-MRC1 antibody (A02285-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: human liver tissue lysates,
Lane 4: monkey lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MRC1 antigen affinity purified polyclonal antibody (A02285-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MRC1 at approximately 190-200 kDa. The expected band size for MRC1 is at 166 kDa.
Figure 2. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
MRC1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 3. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
MRC1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 4. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
MRC1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 5. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
MRC1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 6. IF analysis of CD206/MRC1 using anti-CD206/MRC1 antibody (A02285-2).
CD206/MRC1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD206/MRC1 Antibody (A02285-2) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. IF analysis of CD206/MRC1 using anti-CD206/MRC1 antibody (A02285-2).
CD206/MRC1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-CD206/MRC1 Antibody (A02285-2) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).