欢迎访问博士德生物官网! 服务热线:027-67845390 |
您当前的位置: 首页 > 产品中心 > 一抗 > 多克隆抗体

Anti-CD206/MRC1 Antibody

Rabbit polyclonal antibody

说明书 一键复制产品信息

筛选器: All WB IHC IF ELISA

A02285-2

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Figure 1. Western blot analysis of anti-MRC1 antibody (A02285-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat lung tissue lysates,
    Lane 2: mouse lung tissue lysates,
    Lane 3: human liver tissue lysates,
    Lane 4: monkey lung tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MRC1 antigen affinity purified polyclonal antibody (A02285-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MRC1 at approximately 190-200 kDa. The expected band size for MRC1 is at 166 kDa.

    all(8)
  • Figure 2. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
    MRC1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(8)
  • Figure 3. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
    MRC1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(8)
  • Figure 4. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
    MRC1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(8)
  • Figure 5. IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2).
    MRC1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(8)
  • Figure 6. IF analysis of CD206/MRC1 using anti-CD206/MRC1 antibody (A02285-2).
    CD206/MRC1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD206/MRC1 Antibody (A02285-2) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(8)
  • Figure 7. IF analysis of CD206/MRC1 using anti-CD206/MRC1 antibody (A02285-2).
    CD206/MRC1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-CD206/MRC1 Antibody (A02285-2) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(8)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-CD206/MRC1 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CD206; CLEC13D; Macrophage mannose receptor 1; Mannose receptor; mannose receptor; C type 1; MMR; MRC1
产品类型
Polyclonal
检验物种
human, mouse, rat, monkey
应用范围
WB, IHC, IF, ELISA
基因名称
MRC1
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived human Mannose Receptor/MRC1 recombinant protein (Position: D21-A1140).
计算分子量
166 kDa
实际分子量
190-200 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
The mannose receptor (Cluster of Differentiation 206,CD206) is a C-type lectin primarily present on the surface of macrophages,immature dendritic cells and liver sinusoidal endothelial cells,but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is mapped to 10p12.33. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes,including cell-cell recognition,serum glycoprotein turnover,and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses,bacteria,and fungi so that they can be neutralized by phagocytic engulfment.
Uniprot ID
P22897  
RRID
文献引用格式
CD206/MRC1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02285-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
mannose receptor C-type 1
蛋白名全称
Macrophage mannose receptor 1
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunofluorescence (IF):1:50-400
Enzyme linked immunosorbent assay (ELISA):1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

实验方案>

实验步骤

引用文献(0)>

    相关产品>

    成功添加到购物车

    查看购物车 继续购物

    Anti-CD206/MRC1 Antibody

    筛选器: All WB IHC IF ELISA

    亮暗模式切换
    X
    亮暗模式切换
    X

    在线
    客服

    在线客服
    服务时间:08:30 - 17:30

    点击下列客服在线咨询:

    服务热线

    服务
    热线

    客服服务热线

    027-67845390
    客服服务热线

    扫码

    扫码
    咨询

    微信二维码 微信扫一扫立即咨询
    返回顶部 顶部