Immunohistochemistry (IHC): | 1:50-400 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. IHC analysis of COL4A2 using anti-COL4A2 antibody (A02453-1).
COL4A2 was detected in a paraffin-embedded section of human gastric adenocarcinomar tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COL4A2 Antibody (A02453-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 2. IHC analysis of COL4A2 using anti-COL4A2 antibody (A02453-1).
COL4A2 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COL4A2 Antibody (A02453-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 1. IHC analysis of COL4A2 using anti-COL4A2 antibody (A02453-1).
COL4A2 was detected in a paraffin-embedded section of human gastric adenocarcinomar tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COL4A2 Antibody (A02453-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 2. IHC analysis of COL4A2 using anti-COL4A2 antibody (A02453-1).
COL4A2 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COL4A2 Antibody (A02453-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.