Anti-ADO DyLight 488 Conjugated Antibody

Rabbit polyclonal antibody

说明书

筛选器： All FCM

A02700-Dyl488

100μl ￥2380
货期： 5-7天

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Figure 1. Flow Cytometry analysis of A549 cells using anti-Human ADO antibody (A02700-Dyl488).Overlay histogram showing A549 cells stained with A02700-Dyl488 (Blue line).Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品名称

Anti-ADO DyLight 488 Conjugated Antibody

规格/价格

100μl/2380

指标别称

2 aminoethanethiol dioxygenase; ADO; C10orf22; Cysteamine dioxygenase

产品类型

Polyclonal

检验物种

human

应用范围

FCM

基因名称

ADO

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.

偶联物

DyLight 488

激发/发射光谱

Amax=488nm; Emax=515-545nm

成分

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

纯化方式

Immunogen affinity purified.

浓度

500ug/ml

产品形态

Liquid

保存条件

At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

背景资料

Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

Uniprot ID

Q96SZ5

文献引用格式

ADO DyLight 488 Conjugated Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02700-Dyl488)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

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