Anti-ADAMTS5 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC ELISA

A02802-1

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of ADAMTS5 using anti-ADAMTS5 antibody (A02802-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Human hela whole cell lysates,
Lane 2: Human hepG2 whole cell lysates,
Lane 3: Human U2OS whole cell lysates,
Lane 4: Human SW620 whole cell lysates,
Lane 5: Human THP-1 whole cell lysates,
Lane 6: Human Raji whole cell lysates,
Lane 7: Human SGC-7901 whole cell lysates,
Lane 8: rat liver tissue lysates,
Lane 9: rat testis tissue lysates,
Lane 10: mouse liver tissue lysates,
Lane 11: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ADAMTS5 antigen affinity purified polyclonal antibody (A02802-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ADAMTS5 at approximately 75 kDa. The expected band size for ADAMTS5 is at 102 kDa.
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Figure 2. IHC analysis of ADAMTS5 using anti-ADAMTS5 antibody (A02802-1).
ADAMTS5 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ADAMTS5 Antibody (A02802-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ADAMTS5 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

ADAM metallopeptidase with thrombospondin type 1 motif 5; ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11; ADAMTS-5; ADAMTS11; ADMP-2; ADAMTS5

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ELISA

基因名称

ADAMTS5

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human ADAMTS5 recombinant protein (Position: D747-K780). Human ADAMTS5 shares 100% amino acid (aa) sequence identity with mouse ADAMTS5.

计算分子量

102 kDa

实际分子量

75 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ADAMTS5 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 5), is an enzyme that in humans is encoded by the ADAMTS5 gene. ADAMTS5 is a member of the large ADAMTS family of zinc-dependent proteases. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. By somatic cell hybrid analysis, the human ADAMTS5 gene is mapped to chromosome 21. Used mouse models, it is showed that Sdc4 controls a pathway that activates Adamts5 at the chondrocyte cell surface through Erk1/Erk2 activation of Mmp3.

Uniprot ID

Q9UNA0

文献引用格式

ADAMTS5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02802-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

ADAM metallopeptidase with thrombospondin type 1 motif, 5

蛋白名全称

A disintegrin and metalloproteinase with thrombospondin motifs 5

实验方案>

实验步骤

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