Western blot analysis of CD70 using anti-CD70 antibody (A02853-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: PC-12 whole cell lysates,
Lane 6: B16 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD70 antigen affinity purified polyclonal antibody (A02853-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD70 at approximately 30 kDa. The expected band size for CD70 is at 22 kDa.
Western blot analysis of CD70 using anti-CD70 antibody (A02853-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: RAW264.7 whole cell lysates,
Lane 5: ANA-1 whole cell lysates,
Lane 6: SP2/0 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD70 antigen affinity purified polyclonal antibody (A02853-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD70 at approximately 30 kDa. The expected band size for CD70 is at 22 kDa.
IHC analysis of CD70 using anti-CD70 antibody (A02853-2).
CD70 was detected in a paraffin-embedded section of mouse lymphonode tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD70 Antibody (A02853-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD70 using anti-CD70 antibody (A02853-2).
CD70 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD70 Antibody (A02853-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| ELISA： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of CD70 using anti-CD70 antibody (A02853-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: PC-12 whole cell lysates,
Lane 6: B16 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD70 antigen affinity purified polyclonal antibody (A02853-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD70 at approximately 30 kDa. The expected band size for CD70 is at 22 kDa.
Western blot analysis of CD70 using anti-CD70 antibody (A02853-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: RAW264.7 whole cell lysates,
Lane 5: ANA-1 whole cell lysates,
Lane 6: SP2/0 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD70 antigen affinity purified polyclonal antibody (A02853-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD70 at approximately 30 kDa. The expected band size for CD70 is at 22 kDa.
IHC analysis of CD70 using anti-CD70 antibody (A02853-2).
CD70 was detected in a paraffin-embedded section of mouse lymphonode tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD70 Antibody (A02853-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD70 using anti-CD70 antibody (A02853-2).
CD70 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD70 Antibody (A02853-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of CD70 using anti-CD70 antibody (A02853-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: PC-12 whole cell lysates,
Lane 6: B16 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD70 antigen affinity purified polyclonal antibody (A02853-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD70 at approximately 30 kDa. The expected band size for CD70 is at 22 kDa.
Western blot analysis of CD70 using anti-CD70 antibody (A02853-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: RAW264.7 whole cell lysates,
Lane 5: ANA-1 whole cell lysates,
Lane 6: SP2/0 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD70 antigen affinity purified polyclonal antibody (A02853-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD70 at approximately 30 kDa. The expected band size for CD70 is at 22 kDa.
IHC analysis of CD70 using anti-CD70 antibody (A02853-2).
CD70 was detected in a paraffin-embedded section of mouse lymphonode tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD70 Antibody (A02853-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD70 using anti-CD70 antibody (A02853-2).
CD70 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD70 Antibody (A02853-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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