Anti-AKR1B10 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

A02976

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of AKR1B10 using anti-AKR1B10 antibody (A02976). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKR1B10 antigen affinity purified polyclonal antibody (A02976) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AKR1B10 at approximately 36 kDa. The expected band size for AKR1B10 is at 36 kDa.
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IHC analysis of AKR1B10 using anti-AKR1B10 antibody (A02976).
AKR1B10 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AKR1B10 Antibody (A02976) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of AKR1B10 using anti-AKR1B10 antibody (A02976).
AKR1B10 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AKR1B10 Antibody (A02976) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IF analysis of AKR1B10 using anti-AKR1B10 antibody (A02976).
AKR1B10 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-AKR1B10 Antibody (A02976) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(4)

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产品简介>

产品名称

Anti-AKR1B10 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AKR1B10; AKR1B11; AKR1B12; Aldose reductase like; ALDRLn; ARL 1; ARL1; ARP; hARP; HSI; SI reductase; Small intestine reductase

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, ICC/IF

基因名称

AKR1B10

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human AKR1B10.

计算分子量

36 kDa

实际分子量

36 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Aldo-keto reductase family 1 member B10 is an enzyme that in humans is encoded by the AKR1B10 gene. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis.

Uniprot ID

O60218

文献引用格式

AKR1B10 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02976)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

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