Anti-Laminin gamma 2/LAMC2 Antibody

50μl/1280 100μl/2180 150μl/2800

B2T; BM600; CSF; CSF 140 kDa subunit; EBR2; EBR2A; Epiligrin subunit gamma; Kalinin subunit gamma; Ladsin 140 kDa subunit; LAMB2T; LAMC2; Laminin 5 subunit gamma; Laminin B2t chain; Laminin subunit gamma 2; laminin; gamma 2; LAMNB2; Nicein subunit gamma

Polyclonal

human

WB, ELISA

LAMC2

Rabbit

IgG

E.coli-derived human LAMC2 recombinant protein (Position: A18-S1162).

131 kDa

131 kDa

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

Immunogen affinity purified.

500 ug/ml

Liquid

12 months from date of receipt，-20℃ as supplied.

Laminin gamma2, Laminin subunit gamma-2, is a protein that in humans is encoded by the LAMC2 gene. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma(formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly though to be a truncated version of beta chain(B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5(earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized.

Q13753  

AB_3081621

Laminin gamma 2/LAMC2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A03214)

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

laminin subunit gamma 2

Laminin subunit gamma-2

Western blot (WB):	1:500-2000
Enzyme linked immunosorbent assay (ELISA):	1:100-1000