Anti-LAMC1/LAMC2/LAMC3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF

A03522

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody (A03522). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: rat RH-35 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse Hepa1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 antigen affinity purified polyclonal antibody (A03522) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LAMC1/LAMC2/LAMC3 at approximately 150, 220-250 kDa. The expected band size for LAMC1/LAMC2/LAMC3 is at 177 kDa.
all(8)
IHC analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody (A03522).
LAMC1/LAMC2/LAMC3 was detected in a paraffin-embedded section of mouse heart tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 Antibody (A03522) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody (A03522).
LAMC1/LAMC2/LAMC3 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 Antibody (A03522) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody (A03522).
LAMC1/LAMC2/LAMC3 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 Antibody (A03522) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody (A03522).
LAMC1/LAMC2/LAMC3 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 Antibody (A03522) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IF analysis using anti- Laminin antibody (A03522). detected in paraffin-embedded section of mouse skeletal muscle tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
all(8)
IF analysis using anti- Laminin antibody (A03522). detected in paraffin-embedded section of mouse skeletal muscle tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
all(8)
IF analysis using anti- Laminin antibody (A03522). detected in paraffin-embedded section of rat skeletal muscle tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
all(8)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-LAMC1/LAMC2/LAMC3 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Lamc1; Lamb-2; Lamc-1; Lamc2; Lamc3; Lamc1; Lamc2; Lamc3

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF

基因名称

Lamc1/Lamc2/Lamc3

宿主

Rabbit

抗体亚型

IgG

免疫原

Peptide mixture of laminin gamma1,2,3(NKLNEIEGSLNKAKDEMKAS; DLEERVRRQRNHLHLLETSI; LQLDSHGALHHKLRQLEEES). Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin.

计算分子量

177 kDa

实际分子量

220-250 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. Laminins form independent networks and are associated with type IV collagen networks via entactin, fibronectin, and perlecan. They are important and biologically active parts of the basal lamina, influencing cell differentiation, migration, and adhesion, as well as phenotype and survival. Laminins are trimeric proteins that contain an α-chain, a β-chain, and a γ-chain, found in five, four, and three genetic variants, respectively. Laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival.

Uniprot ID

P02468/Q61092/Q9R0B6

文献引用格式

LAMC1/LAMC2/LAMC3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A03522)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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