Anti-AANAT Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB ELISA

A03784-1

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of AANAT using anti-AANAT antibody (A03784-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse eye tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AANAT antigen affinity purified polyclonal antibody (A03784-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AANAT at approximately 23 kDa. The expected band size for AANAT is at 23 kDa.
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产品简介>

产品名称

Anti-AANAT Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

AA NAT; AANAT; Serotonin acetylase; Serotonin N acetyltransferase; SNAT

产品类型

Polyclonal

检验物种

mouse

应用范围

WB, ELISA

基因名称

Aanat

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse Aanat recombinant protein (Position: C24-C205).

计算分子量

23 kDa

实际分子量

23 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Aralkylamine N-acetyltransferase (AANAT) (EC 2.3.1.87), also known as arylalkylamine N-acetyltransferase or serotonin N-acetyltransferase (SNAT), is an enzyme that is involved in the day/night rhythmic production of melatonin, by modification of serotonin. It is in humans encoded by the ~2.5 kb AANAT gene containing four exons, located on chromosome 17q25. The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternative splicing results in multiple transcript variants.

Uniprot ID

O88816

文献引用格式

AANAT Antibody (Boster Biological Technology, Wuhan, China. Catalog#A03784-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

arylalkylamine N-acetyltransferase

蛋白名全称

Serotonin N-acetyltransferase

实验方案>

实验步骤

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