Figure 1. Western blot analysis of AATF using anti-AATF antibody (A03945-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AATF antigen affinity purified polyclonal antibody (A03945-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AATF at approximately 95 kDa. The expected band size for AATF is at 63 kDa.
Figure 2. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IF analysis of AATF using anti-AATF antibody (A03945-3).
AATF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. IF analysis of AATF using anti-AATF antibody (A03945-3) and anti-Beta Tubulin antibody (M01857-3).
AATF was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.The section was counterstained with DAPI (Catalog # AR1176) (Blue).
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of AATF using anti-AATF antibody (A03945-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AATF antigen affinity purified polyclonal antibody (A03945-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AATF at approximately 95 kDa. The expected band size for AATF is at 63 kDa.
Figure 2. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IF analysis of AATF using anti-AATF antibody (A03945-3).
AATF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. IF analysis of AATF using anti-AATF antibody (A03945-3) and anti-Beta Tubulin antibody (M01857-3).
AATF was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 1. Western blot analysis of AATF using anti-AATF antibody (A03945-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AATF antigen affinity purified polyclonal antibody (A03945-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AATF at approximately 95 kDa. The expected band size for AATF is at 63 kDa.
Figure 2. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of AATF using anti-AATF antibody (A03945-3) .
AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IF analysis of AATF using anti-AATF antibody (A03945-3).
AATF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. IF analysis of AATF using anti-AATF antibody (A03945-3) and anti-Beta Tubulin antibody (M01857-3).
AATF was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-AATF Antibody (A03945-3) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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