Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
Flow Cytometry (Fixed): | 1:50-200 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. Western blot analysis of anti- YWHAG antibody (A04148-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat skeletal muscle tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse skeletal muscle tissue lysates.
Use rabbit anti- YWHAG 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAG at approximately 28KD. The expected band size for YWHAG is at 28KD.
Figure 2. IHC analysis using anti- YWHAG antibody (A04148-2). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. Flow cytometry analysis of U87 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).