Anti-15 Lipoxygenase 2/ALOX15B Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A04331-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ALOX15B using anti-ALOX15B antibody (A04331-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ALOX15B antigen affinity purified polyclonal antibody (A04331-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALOX15B at approximately 76 kDa. The expected band size for ALOX15B is at 76 kDa.
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产品简介>

产品名称

Anti-15 Lipoxygenase 2/ALOX15B Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

15 Lipoxygenase 2; 15 LOX 2; 15 LOX B; ALOX15B; ALOX8; Arachidonate 15 lipoxygenase B

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ELISA

基因名称

ALOX15B

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human ALOX15B recombinant protein (Position: M1-L354).

计算分子量

76 kDa

实际分子量

76 kDa

成分

500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.

Uniprot ID

O15296

文献引用格式

15 Lipoxygenase 2/ALOX15B Antibody (Boster Biological Technology, Wuhan, China. Catalog#A04331-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

arachidonate 15-lipoxygenase type B

蛋白名全称

Arachidonate 15-lipoxygenase B

实验方案>

实验步骤

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