Anti-NCOA4 Antibody

筛选器： All WB FCM ELISA

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  Figure 1. Western blot analysis of NCOA4 using anti-NCOA4 antibody (A04368-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human PC-3 whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: human HL-60 whole cell lysates,
  Lane 5: human HepG2 whole cell lysates,
  Lane 6: human Daudi whole cell lysates,
  Lane 7: human A431 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NCOA4 antigen affinity purified polyclonal antibody (A04368-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NCOA4 at approximately 70 kDa. The expected band size for NCOA4 is at 70 kDa.

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  Figure 2. Western blot analysis of NCOA4 using anti-NCOA4 antibody (A04368-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat live tissue lysates,
  Lane 2: rat RH-35 whole cell lysates,
  Lane 3: mouse liver tissue lysates,
  Lane 4: mouse Hepa1/6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NCOA4 antigen affinity purified polyclonal antibody (A04368-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NCOA4 at approximately 70 kDa. The expected band size for NCOA4 is at 70 kDa.

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  Figure 3. Flow Cytometry analysis of HL-60 cells using anti-NCOA4 antibody (A04368-3).
  Overlay histogram showing HL-60 cells stained with A04368-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCOA4 Antibody (A04368-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Figure 1. Western blot analysis of NCOA4 using anti-NCOA4 antibody (A04368-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human PC-3 whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: human HL-60 whole cell lysates,
  Lane 5: human HepG2 whole cell lysates,
  Lane 6: human Daudi whole cell lysates,
  Lane 7: human A431 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NCOA4 antigen affinity purified polyclonal antibody (A04368-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NCOA4 at approximately 70 kDa. The expected band size for NCOA4 is at 70 kDa.

• 

  Figure 2. Western blot analysis of NCOA4 using anti-NCOA4 antibody (A04368-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat live tissue lysates,
  Lane 2: rat RH-35 whole cell lysates,
  Lane 3: mouse liver tissue lysates,
  Lane 4: mouse Hepa1/6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NCOA4 antigen affinity purified polyclonal antibody (A04368-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NCOA4 at approximately 70 kDa. The expected band size for NCOA4 is at 70 kDa.

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  Figure 3. Flow Cytometry analysis of HL-60 cells using anti-NCOA4 antibody (A04368-3).
  Overlay histogram showing HL-60 cells stained with A04368-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCOA4 Antibody (A04368-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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