Anti-Noggin/NOG Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A04448-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Noggin/NOG using anti-Noggin/NOG antibody (A04448-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A375 whole cell lysates,
Lane 5: human 22RV1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Noggin/NOG antigen affinity purified polyclonal antibody (A04448-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Noggin/NOG at approximately 26 kDa. The expected band size for Noggin/NOG is at 26 kDa.
all(3)
IHC analysis of Noggin/NOG using anti-Noggin/NOG antibody (A04448-1) .
Noggin/NOG was detected in a paraffin-embedded section of uman placenta tissue. The tissue section was incubated with rabbit anti-Noggin/NOG Antibody (A04448-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)
all(3)

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产品简介>

产品名称

Anti-Noggin/NOG Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

NOG; Noggin; SYM1; SYNS1

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, ELISA

基因名称

NOG

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human Noggin recombinant protein (Position: L43-T153).

计算分子量

26 kDa

实际分子量

26 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The secreted polypeptide, encoded by NOG gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse.

Uniprot ID

Q13253

文献引用格式

Noggin/NOG Antibody (Boster Biological Technology, Wuhan, China. Catalog#A04448-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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