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Anti-ARID2 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IP

A05064

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of ARID2 using anti-ARID2 antibody (A05064). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human THP-1 whole cell lysates,
    Lane 2: human Jurkat whole cell lysates,
    Lane 3: human HEL whole cell lysates,
    Lane 4: human MCF-7 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ARID2 antigen affinity purified polyclonal antibody (A05064) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ARID2 at approximately 245 kDa. The expected band size for ARID2 is at 197 kDa.

    all(2)
  • IP analysis of ARID2 using anti-ARID2 antibody (A05064) in MCF-7 whole cell lysate.
    Western blot analysis of ARID2 using anti- ARID2 antibody (A05064).
    Lane 1: MCF-7 whole cell lysates(30ug),
    Lane 2: Rabbit control IgG instead of anti- ARID2 antibody in MCF-7 whole cell lysate,
    Lane 3: anti- ARID2 antibody (2μg) + MCF-7 whole cell lysate (500μg).
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ARID2 antigen affinity purified polyclonal antibody (A05064) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ARID2 at approximately 245 kDa. The expected band size for ARID2 is at 197 kDa.

    all(2)

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产品名称
Anti-ARID2 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
ARID2; BAF200; BRG1 associated factor 200; KIAA1557; p200, Zipzap/p200
产品类型
Polyclonal
检验物种
human
应用范围
WB, IP
基因名称
ARID2
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the N-terminus of human ARID2, identical to the related mouse and rat sequences.
计算分子量
197 kDa
实际分子量
245 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
AT-rich interactive domain-containing protein 2 (ARID2) is a protein that in humans is encoded by the ARID2 gene. It is mapped to 12q12. This gene encodes a member of the AT-rich interactive domain (ARID)-containing family of DNA-binding proteins. Members of the ARID family have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and chromatin structure modification. This protein functions as a subunit of the polybromo- and BRG1-associated factor or PBAF (SWI/SNF-B) chromatin remodeling complex which facilitates ligand-dependent transcriptional activation by nuclear receptors. Mutations in this gene are associated with hepatocellular carcinomas. A pseudogene of this gene is found on chromosome1.
Uniprot ID
Q68CP9  
RRID
文献引用格式
ARID2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A05064)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
ImmunoPrecipitation (IP):1:250-300

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    Anti-ARID2 Antibody

    筛选器: All WB IP

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