Western blot (WB): | 1:500-2000 |
ImmunoPrecipitation (IP): | 1:250-300 |
Western blot analysis of ARID2 using anti-ARID2 antibody (A05064). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ARID2 antigen affinity purified polyclonal antibody (A05064) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ARID2 at approximately 245 kDa. The expected band size for ARID2 is at 197 kDa.
IP analysis of ARID2 using anti-ARID2 antibody (A05064) in MCF-7 whole cell lysate.
Western blot analysis of ARID2 using anti- ARID2 antibody (A05064).
Lane 1: MCF-7 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- ARID2 antibody in MCF-7 whole cell lysate,
Lane 3: anti- ARID2 antibody (2μg) + MCF-7 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ARID2 antigen affinity purified polyclonal antibody (A05064) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ARID2 at approximately 245 kDa. The expected band size for ARID2 is at 197 kDa.
Western blot analysis of ARID2 using anti-ARID2 antibody (A05064). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ARID2 antigen affinity purified polyclonal antibody (A05064) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ARID2 at approximately 245 kDa. The expected band size for ARID2 is at 197 kDa.
IP analysis of ARID2 using anti-ARID2 antibody (A05064) in MCF-7 whole cell lysate.
Western blot analysis of ARID2 using anti- ARID2 antibody (A05064).
Lane 1: MCF-7 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- ARID2 antibody in MCF-7 whole cell lysate,
Lane 3: anti- ARID2 antibody (2μg) + MCF-7 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ARID2 antigen affinity purified polyclonal antibody (A05064) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ARID2 at approximately 245 kDa. The expected band size for ARID2 is at 197 kDa.