Anti-NeuN/RBFOX3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF ELISA

A11954-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of NeuN/RBFOX3 using anti-NeuN/RBFOX3 antibody (A11954-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NeuN/RBFOX3 antigen affinity purified polyclonal antibody (A11954-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NeuN/RBFOX3 at approximately 46-55 kDa. The expected band size for NeuN/RBFOX3 is at 41 kDa.
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IHC analysis of NeuN/RBFOX3 using anti-NeuN/RBFOX3 antibody (A11954-1).
NeuN/RBFOX3 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-NeuN/RBFOX3 Antibody (A11954-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of NeuN/RBFOX3 using anti-NeuN/RBFOX3 antibody (A11954-1).
NeuN/RBFOX3 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-NeuN/RBFOX3 Antibody (A11954-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis using anti- NEUN antibody (A11954-1). detected in paraffin-embedded section of rat brain tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1032).
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all(5)

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产品简介>

产品名称

Anti-NeuN/RBFOX3 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Rbfox3; D11Bwg0517e; Hrnbp3; RNA binding protein fox-1 homolog 3; Fox-1 homolog C; Hexaribonucleotide-binding protein 3; Fox-3; Neuronal nuclei antigen; NeuN antigen

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, IF, ELISA

基因名称

RBFOX3

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse NeuN/Rbfox3 recombinant protein (Position: H28-D304).

计算分子量

41 kDa

实际分子量

46-55 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3) is a protein that in humans is encoded by the RBFOX3 gene. This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Uniprot ID

Q8BIF2

RRID

AB_3081716

文献引用格式

NeuN/RBFOX3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A11954-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

RNA binding protein, fox-1 homolog (C. elegans) 3

蛋白名全称

RNA binding protein fox-1 homolog 3

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实验步骤

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