Anti-CGAS Antibody

筛选器： All WB ICC/IF FCM ELISA

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  Figure 1. Western blot analysis of CGAS using anti-CGAS antibody (A31676-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human Caco-2 whole cell lysates,
  Lane 3: human A431 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CGAS antigen affinity purified polyclonal antibody (A31676-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CGAS at approximately 59 kDa. The expected band size for CGAS is at 59 kDa.

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  Figure 2. IF analysis of CGAS using anti-CGAS antibody (A31676-1).
  CGAS was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-CGAS Antibody (A31676-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 3. Flow Cytometry analysis of THP-1 cells using anti-CGAS antibody (A31676-1).
  Overlay histogram showing THP-1 cells stained with A31676-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CGAS Antibody (A31676-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Figure 4. Flow Cytometry analysis of U937 cells using anti-CGAS antibody (A31676-1).
  Overlay histogram showing U937 cells stained with A31676-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CGAS Antibody (A31676-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Figure 1. Western blot analysis of CGAS using anti-CGAS antibody (A31676-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human Caco-2 whole cell lysates,
  Lane 3: human A431 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CGAS antigen affinity purified polyclonal antibody (A31676-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CGAS at approximately 59 kDa. The expected band size for CGAS is at 59 kDa.

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  Figure 2. IF analysis of CGAS using anti-CGAS antibody (A31676-1).
  CGAS was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-CGAS Antibody (A31676-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 3. Flow Cytometry analysis of THP-1 cells using anti-CGAS antibody (A31676-1).
  Overlay histogram showing THP-1 cells stained with A31676-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CGAS Antibody (A31676-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Figure 4. Flow Cytometry analysis of U937 cells using anti-CGAS antibody (A31676-1).
  Overlay histogram showing U937 cells stained with A31676-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CGAS Antibody (A31676-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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